Isolation and identification of Bifidobacterium from dairy products and screening its antibacterial activity

Abstract

Introduction:

Bifidobacterium is one of the most used probiotic microorganisms in the food industry due to its health-enhancing benefits.

Methods:

In this study, twenty samples from five different dairy products were collected. Bifidobacterium was isolated on Man Rogosa Sharpe (MRS) agar and identified through several tests, including Gram staining, catalase test, oxidase test, triple sugar iron test and testing its susceptibility to mupirocin. Antimicrobial activity against 3 Gram-negative and 1 Gram-positive bacteria which includes Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus respectively was evaluated by disc diffusion method and agar well diffusion method on Mueller-Hinton agar (MHA) medium.

Results:

Out of the 20 isolates from dairy products, 6 were identified as Bifidobacterium. All of the 6 isolates showed some antibacterial activity against Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus, and none against Klebsiella pneumoniae.

Conclusion:

The use of Bifidobacterium spp. as an adjunctive agent to therapeutic pharmaceutical agents is a promising approach against multi-drug resistant bacteria due to its potential for antibacterial activity as observed in this study.